Isolation, Identification and In Vitro Test for the Biocontrol Potential of Trichoderma viride on Fusarium oxysporum f. sp. Lycopersici

Introduction: Tomato production in Ethiopia is challenged by many pests and diseases. Fusarium wilt one of the most important diseases tomato affecting its productivity. Methods: tissue soil samples were collected from farmlands around Aksum town to isolate identify pathogenic species Trichoderma with biocontrol efficacy. Samples processed University Biotechnology laboratory following standard procedures. Results Discussion: Eight five isolates obtained. Six identified as oxysporum, whereas remaining two equiseti circinatum . Detached leaf bioassay F. oxysporum on leaves showed lesion variety, Melka oda. The isolated strains screened for potential against virulent vitro showing highest efficacy was morphologically viride - T. dual culture assay demonstrated that inhibits growth f.sp. lycopersici 76.94% inhibition. Conclusion: prevalent growing covered this study. study an effective agent fsp.